rabbit polyclonal anti il 1a  (Bioss)

Journal: The Journal of investigative dermatology

Article Title: Type I IL-1 receptor mediates IL-1 and intracellular IL-1 receptor antagonist effects in skin inflammation.

doi: 10.1038/sj.jid.5700803

Figure Lengend Snippet: Figure 1. PMA-induced skin inflammation in WT mice. (a) Representative histological sections of acetone (upper panel, original magnification 20) and PMA-treated (lower panel, original magnification 20, scale bar ¼ 500 mm) skin of WT mice are shown. PMA treatment resulted in marked epidermal thickening and increased infiltration of inflammatory cells into the dermis. (b) Results of histological scoring for epidermal thickness (left panel) and dermal inflammatory cell infiltration (right panel) are shown for acetone (white columns; n ¼ 5) and PMA-treated (black columns; n ¼ 8) WT mice. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus acetone-treated WT mice, as assessed by t-test. PMA painting induced a significant increase in both epidermal thickness and inflammatory cell infiltration. (c) Cytokine mRNA expression in the skin was analyzed by RNase protection assay and quantified by phosphorimaging for acetone (white columns; n ¼ 3) and PMA-treated (black columns; n ¼ 8) WT mice. Results are expressed as the ratio of cytokine over L32 mRNA expression. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus acetone- treated WT mice, as assessed by t-test. AU, arbitrary units. IL-1a and IL-1Ra mRNA expression was significantly increased in PMA-treated WT mice. (d) Circulating SAA levels were assessed by ELISA in the serum of acetone (white columns; n ¼ 5) or PMA-treated (black columns; n ¼ 8) WT mice. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus acetone-treated WT mice, as assessed by t-test. Serum SAA was significantly elevated in PMA-treated WT mice.

Article Snippet: Sections were then stained using a rabbit polyclonal anti-IL-1a antibody (sc-7929; Santa Cruz Biotechnologies, Santa Cruz, CA; 1/100) in phosphate-buffered saline containing 1.5% BSA and 5% normal goat serum overnight at 41C.

Techniques: Expressing, Rnase Protection Assay, Enzyme-linked Immunosorbent Assay

Journal: The Journal of investigative dermatology

Article Title: Type I IL-1 receptor mediates IL-1 and intracellular IL-1 receptor antagonist effects in skin inflammation.

doi: 10.1038/sj.jid.5700803

Figure Lengend Snippet: Figure 2. PMA-induced skin inflammation in absence of IL-1 activity. Results of histological scoring for (a) epidermal thickness and (b) dermal inflammatory cell infiltration are shown for PMA-treated WT (n¼ 8), IL-1RI/ (n¼ 5), icIL-1Ra1 tg (n¼ 4), IL-1Ra/ icIL-1Ra1 tg (n¼ 5), IL-1Ra/ IL-1RI/ (n¼ 5) and IL-1Ra/ IL-1RI/ icIL-1Ra1 tg mice (n¼ 8). Results shown represent the mean7SEM for each group of mice. *Po0.05 versus WT mice, as assessed by analysis of variance (ANOVA). Epidermal thickness was similar in all groups of mice, whereas inflammatory cell infiltration was significantly decreased in mice lacking IL-1RI and/or overexpressing icIL-1Ra1, in presence or in absence of endogenous IL-1Ra. (c) IL-1a mRNA expression was analyzed by RNase protection assay and quantified by phosphorimaging in PMA-treated WT (n¼ 8), IL-1RI/ (n¼ 5), icIL-1Ra1 tg (n¼ 4), IL-1Ra/ icIL-1Ra1 tg (n¼ 5), IL-1Ra/ IL-1RI/ (n¼ 5), and IL-1Ra/ IL-1RI/ icIL-1Ra1 tg mice (n¼ 8). Results are expressed as the ratio of IL-1a over L32 mRNA expression. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus WT mice, as assessed by ANOVA. AU, arbitrary units. IL-1a mRNA levels were significantly elevated in icIL-1Ra1 tg and in IL-1Ra/ icIL-1Ra1 tg, as compared with WT mice. (d) Circulating SAA levels were assessed by ELISA in the serum of PMA-treated WT (n¼ 8), IL-1RI/ (n¼ 5), icIL-1Ra1 tg (n¼ 4), IL-1Ra/ icIL-1Ra1 tg (n¼ 5), IL-1Ra/ IL-1RI/ (n¼ 5) and IL-1Ra/ IL-1RI/ icIL-1Ra1 tg mice (n ¼ 8). Results shown represent the mean7SEM for each group of mice. *Po0.05 versus WT mice, as assessed by ANOVA. SAA levels were significantly lower in PMA-treated mice lacking IL-1RI and/or overexpressing icIL-1Ra1, in presence or in absence of endogenous IL-1Ra.

Article Snippet: Sections were then stained using a rabbit polyclonal anti-IL-1a antibody (sc-7929; Santa Cruz Biotechnologies, Santa Cruz, CA; 1/100) in phosphate-buffered saline containing 1.5% BSA and 5% normal goat serum overnight at 41C.

Techniques: Activity Assay, Expressing, Rnase Protection Assay, Enzyme-linked Immunosorbent Assay

Journal: The Journal of investigative dermatology

Article Title: Type I IL-1 receptor mediates IL-1 and intracellular IL-1 receptor antagonist effects in skin inflammation.

doi: 10.1038/sj.jid.5700803

Figure Lengend Snippet: Figure 4. Skin IL-1a and IL-1b mRNA expression and systemic inflammatory response in IL-1Ra/ mice. (a) IL-1a mRNA levels were quantified by RNase protection assay for acetone (white columns; WT, n ¼ 3; IL-1Ra/, n ¼ 5) or PMA-treated (black columns; n ¼ 8 per genotype) WT and IL-1Ra/ mice. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus acetone-treated WT mice, as assessed by ANOVA. AU, arbitrary units. PMA painting resulted in similar changes in IL-1a mRNA expression in WT and IL-1Ra/ mice. (b) IL-1a protein expression was examined by immunohistochemistry in PMA-treated skin of WT (upper left panel, original magnification 20) and IL-1Ra/ mice (lower left panel, original magnification 20). Incubations without the first antibody are shown as negative controls (right panels, original magnification 20, bar ¼ 500 mm). High IL-1a expression was observed in particular in the epidermis. (c) Left panel: a representative RNase protection assay is shown for skin RNA isolated from a PMA-treated WT mouse (lane 1) and an acetone-treated IL-1Ra / mouse (lane 2). The identity of the detected bands is indicated on the left. Middle panel: IL-1b mRNA levels were quantified by RNase protection assay for acetone (white columns; WT, n ¼ 3; IL-1Ra/, n ¼ 5) or PMA-treated (black columns; n ¼ 8 per genotype) WT and IL-1Ra/ mice. Results shown represent the mean7SEM for each group of mice. AU, arbitrary units. Right panel: IL-1b mRNA levels correlated with the severity of macroscopic skin lesions observed on IL-1Ra/ mice (n ¼ 12, P ¼ 0.0015 as assessed by Spearman’s correlation). The graph shows a linear regression of IL-1b mRNA expression levels as a function of macroscopic skin lesion scores (r2 ¼ 0.664). (d) Circulating SAA levels were assessed by ELISA in the serum of acetone (white columns; n ¼ 5 per genotype) or PMA-treated (black columns; n ¼ 8 per genotype) WT and IL-1Ra/ mice. Results shown represent the mean7SEM for each group of mice. *Po0.05 versus acetone-treated WT mice, as assessed by ANOVA. SAA was significantly increased in acetone-treated IL-1Ra/ as compared with WT mice. SAA levels were similar in PMA-treated WT and IL-1Ra/ mice.

Article Snippet: Sections were then stained using a rabbit polyclonal anti-IL-1a antibody (sc-7929; Santa Cruz Biotechnologies, Santa Cruz, CA; 1/100) in phosphate-buffered saline containing 1.5% BSA and 5% normal goat serum overnight at 41C.

Techniques: Expressing, Rnase Protection Assay, Immunohistochemistry, Isolation, Enzyme-linked Immunosorbent Assay